# Top 20 Health Care VC (Venture Capital) Funds in Czech Republic in May 2026

## Reflex Capital
- Type: VC Fund
- Location: Prague, Hlavni mesto Praha, Czech Republic
- Relevant deals: 5
- Stages: Seed, Series A, Pre-Seed
- Focus: Software, SaaS, Information Technology

Doing business may resemble a jungle survival. You better be prepared and have the right reflexes! It's also useful to have someone with real experience around. We at Reflex Capital are entrepreneurs, just like you. We built our own companies, negotiated with investors, and took part in plenty of transactions.

## N1
- Type: VC Fund
- Location: Prague, Hlavni mesto Praha, Czech Republic
- Relevant deals: 5
- Stages: Pre-Seed, Seed
- Focus: Software, Information Technology, Health Care

Nation 1 VC is a Prague and Brno based venture capital firm focused on investing in seed-stage startups operating in the Czech Republic.The firm seeks to invest in the fintech, insurancetech, artificial intelligence, customertech, ecommerce and travel sectors.

## MITON
- Type: VC Fund
- Location: Prague, Hlavni mesto Praha, Czech Republic
- Relevant deals: 5
- Stages: Seed, Pre-Seed
- Focus: E-Commerce, Software, Internet

Company builders operating from Czech Republic and focusing on Central and Eastern Europe. We prefer building consumer facing businesses.

## Purple Ventures
- Type: VC Fund
- Location: Brno, Jihomoravsky kraj, Czech Republic
- Relevant deals: 2
- Stages: Seed, Pre-Seed
- Focus: Medical, Manufacturing, Information Services

We invest smart, kind money into early-stage purpose-driven technology startups with initial revenue and global ambitions.

## DEPO Ventures
- Type: VC Fund
- Location: Prague, Hlavni mesto Praha, Czech Republic
- Relevant deals: 2
- Stages: Pre-Seed, Seed, Series A
- Focus: Software, Artificial Intelligence, Machine Learning

DEPO Ventures is an angel network and VC (Grouport and DEPO Angels Fund II), which connects world of startups and investors.

## Octopus Ventures
- Type: VC Fund
- Location: London, England, United Kingdom
- Relevant deals: 2
- Stages: Series A, Seed, Series B
- Focus: Software, Health Care, FinTech

We provide venture capital investment for the people, ideas and industries that will change the world. Learn about what we do and the kind of founders we back.

## 3VC
- Type: VC Fund
- Location: Vienna, Wien, Austria
- Relevant deals: 2
- Stages: Series A, Seed, Series B
- Focus: Software, Information Technology, SaaS

We believe in entrepreneurs with big ideas  and the determination to drive humanity forward.

## Grouport Ventures
- Type: VC Fund
- Location: Prague, Hlavni mesto Praha, Czech Republic
- Relevant deals: 1
- Stages: Seed, Pre-Seed, Convertible Note
- Focus: SaaS, Apps, Augmented Reality

Grouport is a dynamic and versatile fund with a unique approach – it combines direct engaged involvement in terms of project portfolio with an attractive return on investment. As such, the Investor enters the startup ecosystem and becomes a member of a unique network of fellow Angels investing their financial means and know-how into progressivestartups shaping the 21st century.Further, Grouport allows you to enter the startup ecosystem without the need to abandon or divert from your current job occupation and career.	Grouport – engaged involvement in progressive startups that have the potential to significantly affect our future Grouport – bringing together a remarkable group of people with a common goal and allowing them to grow.Grouport – unique investment opportunity for those seeking new challenges and creating projects from scratch.

## Zero Gravity Capital
- Type: VC Fund
- Location: Bratislava, Bratislava, Slovakia (Slovak Republic)
- Relevant deals: 1
- Stages: Seed, Pre-Seed
- Focus: Artificial Intelligence, Machine Learning, Software

early stage investor

## Tensor Ventures
- Type: VC Fund
- Location: Prague, Hlavni mesto Praha, Czech Republic
- Relevant deals: 1
- Stages: Pre-Seed, Seed
- Focus: Artificial Intelligence, Information Technology, Machine Learning

New generation venture capital investing into combinatorial technologies

## Amazon
- Type: VC Fund
- Location: Seattle, Washington, United States
- Relevant deals: 1
- Stages: Series B, Seed
- Focus: Software, E-Commerce, Information Technology

Amazon is an international e-commerce website for consumers, sellers, and content creators. It offers users merchandise and content purchased for resale from vendors and those offered by third-party sellers.Operating in North American and International markets, Amazon provides its services through websites such as amazon.com and amazon.ca. Italso enables authors, musicians, filmmakers, app developers, and others to publish and sell content via its branded websites.Amazon also provides Kindle Direct Publishing, an online platform that allows independent authors and publishers to make their books available in the Kindle Store. In addition, it provides co-branded credit card agreements and advertising services, serves developers and enterprises through Amazon Web Services, and manufactures and sells electronic devices.It offers Amazon Prime which is an annual membership program. It provides free shipping, streaming, and access to books to borrow and read on a Kindle device.The company was founded in 1994 and is headquartered in Seattle, Washington.

## Presto Ventures
- Type: VC Fund
- Location: Prague, Hlavni mesto Praha, Czech Republic
- Relevant deals: 1
- Stages: Seed, Pre-Seed, Series A
- Focus: Software, SaaS, Information Technology

We're a Prague-based VC firm investing in fast growing tech companies and empowering talented startup founders from Central and Eastern Europe.

## RSJ Investments SICAV a.s.
- Type: VC Fund
- Location: Prague, Hlavni mesto Praha, Czech Republic
- Relevant deals: 1
- Stages: Seed, Series A
- Focus: Artificial Intelligence, 3D Technology, Biotechnology

RSJ Private Equity manages a broad investment portfolio in real estate, energy industry, agriculture, biotechnologies and other economic sectors. We focus on key industries for the 21st century, such as power generation from renewables, sustainable and healthy food production, information technology, and life sciences, applied research leading to alonger and better human life. In addition to considering profitability, we take into account our projects´ potential to contribute to significant shifts in the given industry or field of activity.

## Evolution Equity Partners
- Type: VC Fund
- Location: New York, New York, United States
- Relevant deals: 1
- Stages: Series A, Seed, Series B
- Focus: Cyber Security, Network Security, Software

Evolution Equity Partners is an international venture capital investor leveraging deep sector expertise to help exceptional entrepreneurs develop market-leading companies. The firm has a focus on cyber-security, enterprise software and consumer related opportunities and brings significant resources to portfolio companies seeking to expand ininternational markets. Evolution Equity Partners has invested in well-known companies including AVG Technologies, OpenDNS, Carbon Black, Onapsis, Security Scorecard, Cognitive Security, DF Labs, 60K Group and others. The firm purchases both primary and secondary shares investing in high growth businesses across multiple stages, primarily in North America, Europe and Israel.
Visit us at www.evolutionequity.com or follow us on Twitter at @EvolutionEquity

## BrightCap Ventures
- Type: VC Fund
- Location: Amsterdam, Noord-Holland, The Netherlands
- Relevant deals: 1
- Stages: Seed, Pre-Seed
- Focus: Software, Health Care, SaaS

We invest in early-stage tech startups with roots in Southeast Europe, working on defensible technology and a clear strategy for entering affluent markets.

## Tilia Impact Ventures
- Type: VC Fund
- Location: Prague, Hlavni mesto Praha, Czech Republic
- Relevant deals: 1
- Stages: Pre-Seed, Seed
- Focus: SaaS, Waste Management, B2B

Tilia Impact Ventures (Prague) supports CEE founders addressing meaningful social and environmental problems at scale. We invest across climate, education, health and social inclusion at pre-seed and seed stage.Tilia helps founders to maximise their impact alongside financial growth and we guide early stage impactful startups to the globalinvestment stage.

## Pilulka Lab
- Type: VC Fund
- Relevant deals: 1
- Stages: Pre-Seed
- Focus: Medical, Software, Health Care

: artificial intelligence, medical data, healthcare, blockchain and active life.

## i&i Biotech Fund
- Type: VC Fund
- Location: Luxembourg, Luxembourg, Luxembourg
- Relevant deals: 1
- Stages: Seed, Series A
- Focus: Health Care, Biotechnology, Pharmaceutical

We are i&i Biotech Fund, partners of outstanding founders on their path to global success. We invest in innovative early stage Life Sciences startups.

## Venture Club
- Type: VC Fund
- Relevant deals: 1
- Stages: Seed
- Focus: Pet, InsurTech, Insurance

Venture Club is a private club for investors interested in commonly unavailable investment opportunities, alternative investments, projects and business plans.They are engaged in searching for and selecting suitable projects for investment. They organize investment, educational and social events. They operate an online investment platform fortheir members and manage investments from several tens of thousands of CZK.Venture Club is a gateway to the world of big finance even for smaller investors. The advantage is co-investment with a share of the return, sharing investment tips and a greater number of open opportunities.

## KHAN Technology Transfer Fund
- Type: VC Fund
- Location: Dortmund, Nordrhein-Westfalen, Germany
- Relevant deals: 1
- Stages: Seed, Series A
- Focus: Medical, Therapeutics, Biotechnology

KHAN Technology Transfer Fund operates as an investment firm.

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